Poeciliidae
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- Belonesox belizanus (Belonesox belizanus belizanus?)
- Brachyrhaphis cascajalensis
- Brachyrhaphis episcopi
- Brachyrhaphis hartwegi
- Brachyrhaphis hessfeldi
- Brachyrhaphis holdridgei
- Brachyrhaphis olomina
- Brachyrhaphis parismina
- Brachyrhaphis punctifer
- Brachyrhaphis rhabdophora
- Brachyrhaphis roseni
- Brachyrhaphis roswithae
- Brachyrhaphis terrabensis
- Cnesterodon brevirostratus
- Cnesterodon carnegiei
- Cnesterodon decemmaculatus
- Cnesterodon holopteros
- Cnesterodon hypselurus
- Cnesterodon iguape
- Cnesterodon omorgmatos
- Cnesterodon pirai
- Cnesterodon raddai
- Cnesterodon septentrionalis
- Flexipenis vittata
- Gambusia affinis
- Gambusia alvarezi
- Gambusia amistadensis (extinct)
- Gambusia atrora
- Gambusia aurata
- Gambusia baracoana
- Gambusia beebei
- Gambusia bucheri
- Gambusia caymanensis
- Gambusia clarkhubbsi
- Gambusia echeagarayi
- Gambusia eurystoma
- Gambusia gaigei
- Gambusia geiseri
- Gambusia georgei
- Gambusia heterochir
- Gambusia hispaniolae
- Gambusia holbrooki
- Gambusia howelli
- Gambusia hubbsi
- Gambusia hurtadoi
- Gambusia krumholzi
- Gambusia lemaitrei
- Gambusia longispinis
- Gambusia luma
- Gambusia manni
- Gambusia marshi
- Gambusia melapleura
- Gambusia milleri
- Gambusia monticola
- Gambusia nicaraguensis
- Gambusia nobilis
- Gambusia oligosticta
- Gambusia panuco
- Gambusia pseudopunctata
- Gambusia punctata
- Gambusia puncticulata
- Gambusia quadruncus
- Gambusia rachowi
- Gambusia regani
- Gambusia rhizophorae
- Gambusia senilis
- Gambusia sexradiata
- Gambusia speciosa
- Gambusia vittata
- Gambusia wrayi
- Gambusia xanthosoma
- Gambusia yucatana australis
- Gambusia yucatana yucatana
- Gambusia zarskei
- Girardinus creolus
- Girardinus cubensis
- Girardinus denticulatus
- Girardinus falcatus
- Girardinus metallicus
- Girardinus microdactylus
- Girardinus serripenis
- Girardinus uninotatus
- Heterandria formosa
- Heterandria tuxtlaensis
- Limia caymanensis
- Limia melanogaster
- Limia nigrofasciata
- Limia perugiae
- Limia tridens
- Limia vittata
- Micropoecilia parae
- Micropoecilia picta
- Micropoecilia sp. aff. minima
- Neoheterandria elegans
- Phallichthys amates
- Phallichthys fairweatheri
- Phallichthys quadripunctatus
- Phallichthys tico
- Phalloceros caudimaculatus
- Phalloceros caudimaculatus reticulatus
- Poecilia caucana
- Poecilia mexicana mexicana
- Poecilia obscura
- Poecilia reticulata
- Poecilia salvatoris
- Poecilia sp.
- Poecilia sphenops
- Poecilia vandepolli
- Poecilia wingei
- Poeciliopsis infans
- Priapella chamulae
- Priapella compressa
- Priapella intermedia
- Priapichthys annectens
- Scolichthys iota
- Xenophallus umbratilis
- Xiphophorus alvarezi
- Xiphophorus clemenciae
- Xiphophorus continens
- Xiphophorus cortezi
- Xiphophorus couchianus
- Xiphophorus evelynae
- Xiphophorus helleri
- Xiphophorus maculatus
- Xiphophorus mayae
- Xiphophorus milleri
- Xiphophorus montezumae
- Xiphophorus nezahualcoyotl
- Xiphophorus nigrensis
- Xiphophorus pygmaeus
- Xiphophorus signum
- Xiphophorus variatus
- Xiphophorus xiphidium